60S ribosomal subunit bound to the E3-UFM1 complex (native, UFM1 pulldown)
DaRosa PA, Penchev I, Gumbin SC, Scavone F, Wachalska M, Paulo JA, Ordureau A, Peter JJ, Kulathu Y, Harper JW, Becker T, Beckmann R, Kopito RR
Nature (2024) 627 pp. 445-452 [ Pubmed: 38383785 DOI: doi:10.1038/s41586-024-07073-0 ]
60S ribosomal subunit bound to the E3-UFM1 complex - state 3 (native)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8ohd
Q-score: 0.531
DaRosa PA, Penchev I, Gumbin SC, Scavone F, Wachalska M, Paulo JA, Ordureau A, Peter JJ, Kulathu Y, Harper JW, Becker T, Beckmann R, Kopito RR
Nature (2024) 627 pp. 445-452 [ Pubmed: 38383785 DOI: doi:10.1038/s41586-024-07073-0 ]
- 60s ribosomal protein l18a (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l21 (18 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23a (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l3 (46 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37 (11 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l29 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l30 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l22 (14 kDa, Protein from Homo sapiens)
- Ribosomal protein ul16-like (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l39 (6 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l4 (47 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- E3 ufm1-protein ligase 1 (Complex from Homo sapiens)
- 60s ribosomal subunit in complex with e3-ufm1 ligase (Complex)
- 5.8s rrna (50 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l19 (23 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l34 (13 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l24 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l32 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l38 (8 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l9 (21 kDa, Protein from Homo sapiens)
- E3 ufm1-protein ligase 1 (89 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal subunit (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l28 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l31 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7 (29 kDa, Protein from Homo sapiens)
- Ddrgk domain-containing protein 1 (35 kDa, Protein from Homo sapiens)
- Cdk5 regulatory subunit-associated protein 3 (56 kDa, Protein from Homo sapiens)
- Ubiquitin-fold modifier 1 (9 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l11 (20 kDa, Protein from Homo sapiens)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27a (16 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l15 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l26 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l10a (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l17 (21 kDa, Protein from Homo sapiens)
- 5s rrna (38 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l5 (34 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37a (10 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l6 (32 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13a (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l14 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7a (30 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l8 (28 kDa, Protein from Homo sapiens)
- 28s rrna (1 MDa, RNA from Homo sapiens)
60S ribosomal subunit bound to the E3-UFM1 complex - state 2 (native)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8oj0
Q-score: 0.463
DaRosa PA, Penchev I, Gumbin SC, Scavone F, Wachalska M, Paulo JA, Ordureau A, Peter JJ, Kulathu Y, Harper JW, Becker T, Beckmann R, Kopito RR
Nature (2024) 627 pp. 445-452 [ Pubmed: 38383785 DOI: doi:10.1038/s41586-024-07073-0 ]
- 60s ribosomal protein l18a (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l21 (18 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23a (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l3 (46 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37 (11 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l34 (13 kDa, Protein from Homo sapiens environmental sample)
- 60s ribosomal protein l29 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l30 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7a (30 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l22 (14 kDa, Protein from Homo sapiens)
- Ribosomal protein ul16-like (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l39 (6 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l4 (47 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- E3 ufm1-protein ligase 1 (Complex from Homo sapiens)
- 60s ribosomal protein l19 (23 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l24 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l32 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l38 (8 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit beta (9 kDa, Protein from Homo sapiens)
- E3 ufm1-protein ligase 1 (89 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l9 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal subunit bound to the e3-ufm1 complex and sec61 (Complex)
- 60s ribosomal subunit (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l28 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l31 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7 (29 kDa, Protein from Homo sapiens)
- Ddrgk domain-containing protein 1 (35 kDa, Protein from Homo sapiens)
- Cdk5 regulatory subunit-associated protein 3 (56 kDa, Protein from Homo sapiens)
- Ubiquitin-fold modifier 1 (9 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l11 (20 kDa, Protein from Homo sapiens)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27a (16 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l15 (24 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l26 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l10a (24 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit alpha isoform 1 (52 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l17 (21 kDa, Protein from Homo sapiens)
- 5s rrna (38 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l5 (34 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37a (10 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l6 (32 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13a (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l14 (23 kDa, Protein from Homo sapiens)
- 5.8s rrna (50 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l8 (28 kDa, Protein from Homo sapiens)
- 28s rrna (1 MDa, RNA from Homo sapiens)
60S ribosomal subunit bound to the E3-UFM1 complex - state 3 (in-vitro reconstitution)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8oj5
Q-score: 0.57
DaRosa PA, Penchev I, Gumbin SC, Scavone F, Wachalska M, Paulo JA, Ordureau A, Peter JJ, Kulathu Y, Harper JW, Becker T, Beckmann R, Kopito RR
Nature (2024) 627 pp. 445-452 [ Pubmed: 38383785 DOI: doi:10.1038/s41586-024-07073-0 ]
- 60s ribosomal protein l18a (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l21 (18 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36a (12 kDa, Protein from Homo sapiens)
- E3-ufm1 complex bound to the 60s ribosome (Complex from Homo sapiens)
- 60s ribosomal protein l23a (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l3 (46 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37 (11 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l29 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l30 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l22 (14 kDa, Protein from Homo sapiens)
- Ribosomal protein ul16-like (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l39 (6 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l4 (47 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 5.8s rrna (50 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l19 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l34 (13 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l24 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l32 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l38 (8 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l9 (21 kDa, Protein from Homo sapiens)
- E3 ufm1-protein ligase 1 (89 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23 (14 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l28 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l31 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7 (29 kDa, Protein from Homo sapiens)
- Ddrgk domain-containing protein 1 (35 kDa, Protein from Homo sapiens)
- Cdk5 regulatory subunit-associated protein 3 (56 kDa, Protein from Homo sapiens)
- Ubiquitin-fold modifier 1 (9 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l11 (20 kDa, Protein from Homo sapiens)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27a (16 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l15 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l26 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l10a (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l17 (21 kDa, Protein from Homo sapiens)
- 5s rrna (38 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l5 (34 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37a (10 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l6 (32 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13a (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l14 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7a (30 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l8 (28 kDa, Protein from Homo sapiens)
- 28s rrna (1 MDa, RNA from Homo sapiens)
60S ribosomal subunit bound to the E3-UFM1 complex - state 1 (native)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8oj8
Q-score: 0.468
DaRosa PA, Penchev I, Gumbin SC, Scavone F, Wachalska M, Paulo JA, Ordureau A, Peter JJ, Kulathu Y, Harper JW, Becker T, Beckmann R, Kopito RR
Nature (2024) 627 pp. 445-452 [ Pubmed: 38383785 DOI: doi:10.1038/s41586-024-07073-0 ]
- 60s ribosomal protein l18a (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13 (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l21 (18 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23a (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l3 (46 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37 (11 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l29 (17 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit alpha isoform 1 (52 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l30 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l22 (14 kDa, Protein from Homo sapiens)
- Ribosomal protein ul16-like (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l39 (6 kDa, Protein from Homo sapiens)
- 60s ribosomal subunit in complex with e3-ufm1 ligase, state 1 (Complex)
- 60s ribosomal protein l4 (47 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- E3 ufm1-protein ligase 1 (Complex from Homo sapiens)
- 5.8s rrna (50 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l19 (23 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l34 (13 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l24 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l32 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l38 (8 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l9 (21 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit beta (9 kDa, Protein from Homo sapiens)
- E3 ufm1-protein ligase 1 (89 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l23 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal subunit (Complex from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l28 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l31 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7 (29 kDa, Protein from Homo sapiens)
- Ubiquitin-60s ribosomal protein l40 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l11 (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27a (16 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l15 (24 kDa, Protein from Homo sapiens)
- Protein transport protein sec61 subunit gamma (7 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l26 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l27 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l36 (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l10a (24 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l17 (21 kDa, Protein from Homo sapiens)
- 5s rrna (38 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l5 (34 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l37a (10 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35 (14 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l6 (32 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l13a (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l14 (23 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7a (30 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l8 (28 kDa, Protein from Homo sapiens)
- 28s rrna (1 MDa, RNA from Homo sapiens)
Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR
EMBO J (2024) 43 pp. 484-506 [ Pubmed: 38177497 DOI: doi:10.1038/s44318-023-00010-3 ]
Cryo-EM reconstruction of Bacillus subtilis collided disome binding MutS2 SMR and KOW domains
Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR
EMBO J (2024) 43 pp. 484-506 [ Pubmed: 38177497 DOI: doi:10.1038/s44318-023-00010-3 ]
Bacillus subtilis MutS2-collided disome complex (stalled 70S)
Resolution: 3.57 Å
EM Method: Single-particle
Fitted PDBs: 8r55
Q-score: 0.386
Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR
EMBO J (2024) 43 pp. 484-506 [ Pubmed: 38177497 DOI: doi:10.1038/s44318-023-00010-3 ]
- 30s ribosomal protein s11 (13 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s19 (10 kDa, Protein from Bacillus subtilis)
- Mrna (33-mer) (10 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s2 (28 kDa, Protein from Bacillus subtilis)
- 23s rna (2887-mer) (949 kDa, RNA from Bacillus subtilis)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Bacillus subtilis)
- 5s rrna (112-mer) (36 kDa, RNA from Bacillus subtilis)
- Endonuclease muts2 (87 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s18 (8 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s10 (11 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s16 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s20 (9 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl27 (8 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s4 (22 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s3 (24 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s5 (17 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s13 (13 kDa, Protein from Bacillus subtilis)
- B. subtilis endonuclease muts2 binding the collided ribosome interface (Complex from Bacillus subtilis)
- Trna (73-mer) (23 kDa, RNA from Bacillus subtilis)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s6 (11 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul2 (30 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s12 (15 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s8 (14 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s7 (17 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul6 (19 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis)
- Muts2-disome complex from b. subtilis, focus refined, collided 70s (Complex from Bacillus subtilis)
- Trna (77-mer) (24 kDa, RNA from Bacillus subtilis)
- 16s rrna (1533-mer) (496 kDa, RNA from Bacillus subtilis)
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl31 (7 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s9 (14 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s17 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul24 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl33 (5 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl17 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl28 (6 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s15 (10 kDa, Protein from Bacillus subtilis)
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8pv8
Q-score: 0.559
Thoms M, Lau B, Cheng J, Fromm L, Denk T, Kellner N, Flemming D, Fischer P, Falquet L, Berninghausen O, Beckmann R, Hurt E
EMBO Rep (2023) 24 pp. e57984-e57984 [ DOI: doi:10.15252/embr.202357984 Pubmed: 37921038 ]
- 60s ribosomal protein l26-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l28-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l9-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Duf2423 domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l16-like protein (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l32-like protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pre-rrna-processing protein rix1 (84 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 26s rrna (1 MDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein el39 (6 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5.8s rrna (50 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l22-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l13 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l8 (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein rlp24 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l43-like protein (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l37 (10 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l18-like protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Large ribosomal subunit protein ul10 (30 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Protein sda1 (86 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l2-like protein (27 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Water (18 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Putative ribosomal protein (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l27 (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l19 (317 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Zinc finger domain-containing protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l34-like protein (13 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pre-rrna-processing protein ipi3 (47 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Rrna-processing protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l20 (20 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l12-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (Complex from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 1 (75 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l29 (7 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l7-like protein (28 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l17-like protein (21 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l23-like protein (14 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome biogenesis protein nsa2 homolog (29 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Dolichyl-diphosphooligosaccharide--protein glycotransferase (105 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l5-like protein (35 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l4-like protein (39 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein l15 (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l25-like protein (17 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l33-like protein (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome assembly protein 4 (57 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l14-like protein (16 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l21-like protein (18 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative gtp binding protein (61 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Chaetomium thermophilum pre-60s state 2 - pre-5s rotation with rix1 complex - composite structure (Complex)
- 60s ribosomal protein l6 (22 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Ribosome (Complex from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l3-like protein (44 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 5s rrna (38 kDa, RNA from Thermochaetoides thermophila DSM 1495)
- Ribosomal protein (24 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l36 (12 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Nucleolar gtp-binding protein 2 (70 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Pre-rrna-processing protein ipi1 (37 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l30-like protein (11 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- Putative 60s ribosomal protein (15 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l41-a (3 kDa, Protein from Thermochaetoides thermophila DSM 1495)
- 60s ribosomal protein l38-like protein (9 kDa, Protein from Thermochaetoides thermophila DSM 1495)
Bacillus subtilis MutS2-collided disome complex (stalled 70S)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8qpp
Q-score: 0.415
Park EN, Mackens-Kiani T, Berhane R, Esser H, Erdenebat C, Burroughs AM, Berninghausen O, Aravind L, Beckmann R, Green R, Buskirk AR
EMBO J (2024) 43 pp. 484-506 [ Pubmed: 38177497 DOI: doi:10.1038/s44318-023-00010-3 ]
- 30s ribosomal protein s11 (13 kDa, Protein from Bacillus subtilis)
- Muts2-disome complex from b. subtilis, focus refined, stalled 70s (Complex from Bacillus subtilis)
- 30s ribosomal protein s19 (10 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s2 (28 kDa, Protein from Bacillus subtilis)
- Mrna (33-mer) (10 kDa, RNA from Bacillus subtilis)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Bacillus subtilis)
- Endonuclease muts2 (87 kDa, Protein from Bacillus subtilis)
- 5s rrna (112-mer) (36 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s18 (8 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s10 (11 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s16 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s20 (9 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl27 (8 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l9 (16 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s4 (22 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s3 (24 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s5 (17 kDa, Protein from Bacillus subtilis)
- 23s ribosomal rna (949 kDa, RNA from Bacillus subtilis)
- 30s ribosomal protein s13 (13 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s6 (11 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul2 (30 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s12 (15 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s8 (14 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s7 (17 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul6 (19 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis)
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis)
- Trna (77-mer) (24 kDa, RNA from Bacillus subtilis)
- 16s rrna (1533-mer) (496 kDa, RNA from Bacillus subtilis)
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl31 (7 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s9 (14 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s17 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul24 (10 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl33 (5 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl17 (13 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Bacillus subtilis)
- Large ribosomal subunit protein bl28 (6 kDa, Protein from Bacillus subtilis)
- 30s ribosomal protein s15 (10 kDa, Protein from Bacillus subtilis)